b-Cyclodextrin polymer as the immobilization matrix for peroxidase and mediator in the fabrication of a sensor for hydrogen peroxide

A novel immobilization approach based on the supramolecular function between b-cyclodextrin polymer (b-CDP) and cationic dyes, and the condensation polymerization among b-CDP, glutaric dialdehyde and horseradish peroxidase (HRP) in the fabrication of a hydrogen peroxide sensor is described. IR and UV–vis spectroscopy were employed to characterize the structure of the composite membrane modified on the surface of the electrode. AFM was used to visualize the morphology of the composite membrane. The characteristics of supramolecular inclusion compounds between b-CDP and cationic dyes were studied. The fabricated H2O2 sensor responded rapidly to H2O2 in the linear range from 1.0 to 1.1 mmol l −1 with a detection limit of 0.5 mol l. © 2000 Elsevier Science S.A. All rights reserved.